Viabilty Test of Fish Scale Collagen (Oshpronemus Gouramy) on Bone Marrow Mesenchymal Stem Cell Culture
نویسندگان
چکیده
The role of type I collagen is as a matrix extracellular proteins with characteristics increased cell proliferation which directly affects the physiology and morphology cells. Type 1 can be obtained either from fish scales. This what underlies author to support engineering tissue used for treatment periodontal disease in regenerative field by utilizing derived gouramy As an initial step, researchers wanted conduct study using extract scales (Osphoronemus gouramy) was applied bone marrow mesenchymal stem cultures see viability vitro. To determine carp (Osphronemus goramy) Bone cells are taken mice planted 96 well plates. Collagen extracted enzymatic method dissolved condition medium hydrolyzed into hydrolysis solution each concentration 0.01 mg / ml, 0.02 0.04 0.16 0.32 ml put prepared incubated 24 hours MTT assay. increase percentage above 50% highest at 0,01 ml. soaked has better than carp. viable against had optimum dose
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ژورنال
عنوان ژورنال: Journal of Stem Cell Research and Tissue Engineering
سال: 2022
ISSN: ['2614-1256', '2614-1264']
DOI: https://doi.org/10.20473/jscrte.v6i1.37515